Translating Proteomics Episode 9 – Are Proteins a Myth? With Special Guest Neil Kelleher

Chapters

• 00:00 – Introduction to proteoforms
• 03:36 – Why the term “proteoform” was coined
• 11:55 – Are proteins a myth?
• 13:39 – Evidence for the importance of proteoforms
• 22:45 – The Human Proteoform Atlas and Project
• 30:09 – How many proteoforms are there?
• 33:32 – Technological developments required for routine proteoform analysis
• 41:45 – Takeaways with Parag and Andreas

Examples of the power of proteoforms covered in this episode

• Recent work from Neil’s lab showing blood proteoforms can help predict liver transplant success (Melani et al. 2022).
• Work form Ying Ge’s lab showing changes in troponin proteoforms correlate with varying degrees of heart disease (Zhang et al. 2011).
• The BioTyper – a mass spectrometry-based device that can identify different kinds of microbes.

Additional proteoform resources

• The Human Proteoform Atlas webpage
• Publication describing The Human Proteoform Atlas (Hollas et al. 2022)
• Publication discussing how many human proteoforms there are (Aebersold et al. 2018)
• Animation – Proteoform Analysis on the Nautilus Proteome Analysis Platform